MR: We understood that the study would attract attention, but we think it is self-evident that this type of research should be pursued. Let's review.\r\rIf you like this content and want more, I am doing a special lifetime membership offer. They used different concentrations of the vaccine and Serves: 4 Prep. Salesforce has already slashed 8,000 workers from its ranks this year. "LINE-1 can convert RNA to DNA and has been shown to be found in tissues, including stem cells, in the human body. selenagomez. Its precisely what health experts and fact-checkers said for more than a year could not occur. doi: 10.1038/s41586-020-2639-4. No information in this video should ever be considered as a substitute for advice from a healthcare professional.\r\rURL list from Friday, Feb. 25 2022\r\rCIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML\rhttps://www.mdpi.com/1467-3045/44/3/73/htm\r\rFrontiers | Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines | Genetics\rhttps://www.frontiersin.org/articles/10.3389/fgene.2020.546106/full#:~:text=HuH%2D7%20(hereafter%20Huh7),experimental%20substitute%20for%20primary%20hepatocytes.\r\rURL list from Monday, Feb. 28 2022\r\rCIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML\rhttps://www.mdpi.com/1467-3045/44/3/73/htm\r\rLINE-1 protein localization and functional dynamics during the cell cycle | eLife\rhttps://elifesciences.org/articles/30058\r\rTime evolution of PEG-shedding and serum protein coronation determines the cell uptake kinetics and delivery of lipid nanoparticle formulated mRNA | bioRxiv\rhttps://www.biorxiv.org/content/10.1101/2021.08.20.457104v1\r\rReverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues - PubMed\rhttps://pubmed.ncbi.nlm.nih.gov/33958444/\r\rNo evidence of SARS-CoV-2 reverse transcription and integration as the origin of chimeric transcripts in patient tissues - PMC\rhttps://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC8379926/\r\rOverview of Reverse Transcription - Retroviruses - NCBI Bookshelf\rhttps://www.ncbi.nlm.nih.gov/books/NBK19424/\r\rNuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus | Nature Microbiology\rhttps://www.nature.com/articles/s41564-020-0735-8\r\rChimeric RNA - Wikipedia\rhttps://en.wikipedia.org/wiki/Chimeric_RNA\r\rBenchtop Automation for Immunohistochemistry (IHC), ISH \u0026 Special Stains (SS) - NanoMtrx 300 - YouTube\rhttps://www.youtube.com/watch?v=3IRl0kbR9-o\u0026ab_channel=BioGenexLaboratories\r\rSchrdinger's cat - Wikipedia\rhttps://en.wikipedia.org/wiki/Schr%C3%B6dinger%27s_cat The study tested whether the vaccines The. Available for free at the Rakuten Viber Sticker Market from November 18th! RNA levels of BNT162b2 are presented as logged 2, Immunohistochemistry of Huh7 cells treated with BNT162b2 on LINE-1 protein distribution. 3401 Civic Center Blvd. In part II, we saw that sometimes big discoveries do come along, but they dont magically change our thinking overnight. Click here:\rhttps://www.drbeen.com/yt-special-p/\r\rWant to support this work? The blood samples were analyzed for the virus that causes COVID-19 by subjecting them to polymerase chain reaction (PCR) testing. See here for a complete list of exchanges and delays. In the cell line in a petri dish, you dont have those checks in place.. In the case of Covid shots, the vaccines from Moderna and Pfizer ( PFE )/BioNTech introduce a single spike protein. Side Effects. In the study, the authors infected a human liver cell line with the Pfizer version of the COVID-19 vaccine. However, this is not an appropriate conclusion from this paper for several reasons: Logical fallacy called hasty generalization (It is important to note in this case, that the misconception was not because of the quality of the science or the messaging of the authors, but rather because others took the findings out of context.). Suggested accounts. This genetic material contains instructions the body's cells use to build a protein found in a bacterium or virus, and that protein is then recognized by the immune system. Our monetization model empowers Citizen Journalists to report the news in local and national markets. The vaccines mRNA converting into DNA and being found inside the cells nucleus is something that the Centers for Disease Control and Prevention (CDC) said would not happen. 19.9M. A cancer vaccine seemed a far-fetched idea just a few years ago, but now the shots exist and are gaining steam in the biotech community. We have a new vaccine, and it needs to be tested in cell and animal models and also in humans, in various ways. They are not sure if it gets integrated in our DNA or not. The experiment was conducted in a petri dish and aimed to answer the question of whether the mRNA in the vaccine could be converted to DNA. eCollection 2022. WebRead about Pfizer Vaccine Becomes DNA in Liver Cells. Suggested accounts. Original video "Pfizer Vaccine Becomes DNA in Liver Cells. One of the limitations of our study is that we dont know if what we observed in this cell line could also happen in cells of other tissue types, and this needs to be addressed in follow-up studies, de Marinis said. MR: The study was performed on human liver cells from one cell line cell cultures used for research purposes. This is part of APs effort to address widely shared misinformation, including work with outside companies and organizations to add factual context to misleading content that is circulating online. Further, the newest vaccines are personalized, meaning they can target a patients Lund University addressed the misinterpretations circulating on social media (here), featuring the authors of the study, Associate Professor Yang de Marinis and Professor Magnus Rasmussen. These insects are generally larger in temperate areas than in the tropics. Antibody levels were also measured in separate tests. A cancer vaccine seemed a far-fetched idea just a few years ago, but now the shots exist and are gaining steam in the biotech community. Epub 2021 Nov 10. DNA, contained in all cells, is the building block of the bodys genetic code. For general inquiries, please use our contact form. This is 1 replication competent plasmid per 350 mRNA molecules and equates to billions of antibiotic resistant plasmids injected per person per shot . RNA is closely related to DNA, and one type, called messenger RNA or mRNA, sends instructions to the cell for different purposes. The researchers write that the findings warrant a further And even if there was, what are you going to do with 147 kids' data?7 Use this form if you have come across a typo, inaccuracy or would like to send an edit request for the content on this page. Brighter days are ahead and a vaccine is coming! 2022 Dec 7;44(4):72. doi: 10.1007/s40656-022-00548-1. All quotes delayed a minimum of 15 minutes. An official website of the United States government. :\rBuy me a coffee :-) https://www.buymeacoffee.com/DrMobeenSyed\rBecome my patron: https://www.patreon.com/mobeensyed?fan_landing=true\rPayPal: https://paypal.me/mobeensyed?locale.x=en_US\r\r#drbeen #koolbeens #COVID\r\rDisclaimer:\rThis video is not intended to provide assessment, diagnosis, treatment, or medical advice; it also does not constitute provision of healthcare services. Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the BNT162b2 injection. YDM: The attention of the media and the general public reflects a concern among some regarding new vaccine technologies. In thisstudywe present evidence that COVID-19 mRNA vaccine BNT162b2 is able to enter the human liver cell line Huh7 in vitro, the researchers wrote in the study, published inCurrent Issues of Molecular Biology. In order to create these cell lines, the genetic make-up of the cells has to be fiddled with to make them immortal and keep them alive in the petri dish, Dr. David Strain, a senior clinical lecturer at Englands University of Exeter Medical School, wrote in an email to the AP. J. Med. Its And, while this may be the case early during infection, there are a few additional considerations when thinking about these data: Hopefully these examples demonstrated some of the ways that a paper should be examined. Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the -. The American Association of Liver Disease has recently published consensus statements for COVID-19 vaccination in subjects with chronic liver disease (CLD). and Terms of Use. followers 26 videos. The content provided in this video is for informational and educational purposes only.\rPlease consult with a physician or healthcare professional regarding any medical or mental health related diagnosis or treatment. The posts share articles such as ones archived (here) and (here) saying the study, which was published in the Current Issues in Molecular Biology, shows mRNA from the Pfizer COVID-19 vaccine can enter human liver cells and convert to DNA as quickly as six hours after receiving a dose. Coronaviruses, however, are not expected to do this, said Bethany Moore, chair of the University of Michigans Department of Microbiology and Immunology. Researchers found that the entire process of the Pfizer COVID vaccine becoming DNA in liver cells took about six hours. YDM: More research is needed. Nature. Accessibility Safety and Immunogenicity of Two RNA-Based COVID-19 Vaccine Candidates. About BVHS; Mayo Clinic Care Network; News & Media; Contact Us The authors of a Swedish study said their study could not be used to draw conclusions with human DNA as they used cell lines in a laboratory petri dish for their work. Yau K, Chan CT, Abe KT, Jiang Y, Atiquzzaman M, Mullin SI, Shadowitz E, Liu L, Kostadinovic E, Sukovic T, Gonzalez A, McGrath-Chong ME, Oliver MJ, Perl J, Leis JA, Bolotin S, Tran V, Levin A, Blake PG, Colwill K, Gingras AC, Hladunewich MA. Second, they measured LINE-1 activity. Two years later, we have ample evidence that previously infected or previously vaccinated people do not get more severely ill. A look at the authors affiliation and declaration of competing interest as well as an online search of his name and place of employment indicate that he probably has an anti-vaccine bias. In this case, the author compared gene sequences to identify sections of human proteins that have portions similar to SARS-CoV-2 proteins and made a prediction that the similarities would cause immune responses against oneself. Lund Universitymagnus [dot] rasmussen [at] med [dot] lu [dot] se, Yang de Marinis Both LT recipients and HCWs who had developed SARS-CoV-2 infection before vaccination, patients aged under 18, and patients who The clip comes from a longer roundtable discussion that was posted March 8 by Christian broadcasting network Daystar, during which faith leaders discussed COVID-19 vaccines with the doctors. BNT: BNT162b2; L: DNA ladder; Ctrl1: cultured Huh7 cells; Ctrl2: Huh7 cells without BNT162b2 treatment collected at 6 h; Ctrl3: Huh7 cells without BNT162b2 treatment collected at 24 h; Ctrl4: Huh7 cells without BNT162b2 treatment collected at 48 h; Ctrl5: RNA from Huh7 cells treated with 0.5 g/mL of BNT162b2 for 6 h; Ctrl6: RNA from Huh7 cells treated with 0.5 g/mL of BNT162b2 for 6 h, digested with RNase. As long as the spike protein can be detected on cell-derived membrane vesicles, the immune system will be attacking the cells that release these vesicles, they said. N. Engl. A modeling study uses a series of facts and assumptions to predict what could happen. See this image and copyright information in PMC. In part I, we focused on the big picture. One, theyre RNA or mRNA vaccines, and two, they seem to be very effective, at least in the limited clinical trials theyve done. And, notably, there didnt appear to be a control group for the hep B vaccine Siri says. This study of 218 people infected with COVID-19 compared their vaccination status, severity of illness and blood samples. Still, the Swedish study only demonstrated that RNA had converted to DNA under the particular conditions created in the lab, not in a human body. Moore also cautioned that the cells used in the study were quite different than primary cells in the body. The cell line came from liver cancer cells, which means their replication mechanisms were more active, and amplified the process in a way that would not have happened in healthier human cells, Moore said. Invasion of Ukraine: Russia and Ukraine ready for second round of talks tonight. Further proof with COVID-19. Chia PY, Ong SWX, Chiew CJ, et al. Imaging findings included inflammation, beading, stricturing, and dilation of the biliary tree. RNA was purified and qPCR was performed using primers targeting BNT162b2. (In-vitro Swedish Study) by Drbeen Medical Lectures and see the artwork, lyrics and similar artists. First, cancerous cell lines replicate, whereas our liver cells typically are not replicating. Pfizers COVID trials6 enrolled 46,331 participants in a Phase 3 clinical trial while Recombivax HB tested just 147 infants and children. Some viruses, like HIV, are known to be able to convert RNA to DNA and then incorporate that DNA into host cells genome, which is one thing that makes the illness difficult to treat. A new Swedish study published in MDPI found that the Pfizer vaccine goes into liver cells and converts to DNA, challenging claims so far that the mRNA COVID-19 vaccines do not change or interact Shakira. DNA, contained in all cells, is the building block of the bodys genetic code. Experts, and the study authors, say the research is being misinterpreted. (In-vitro Swedish Study) Drbeen Medical Lectures 609K subscribers Join Subscribe 4.3M views Streamed 11 The spike protein naturally occurs on the outside of the virus that causes Covid. (In-vitro Swedish Study) An accepted and published Swedish study demonstrates for the The CDC says that the COVID-19 vaccines do not change or interact with your DNA in any way, claiming that all of the ingredients in both mRNA and viral vector COVID-19 vaccines (administered in the United States) are discarded from the body once antibodies are produced. That is why it is impossible to alter your DNA with mRNA vaccines. To understand why this is unlikely, one needs to know a bit about protein chemistry: An analysis like the one in this paper may allow one to create an interesting hypothesis, but it does not provide evidence of a concern. Knowing that it is theoretically possible for RNA from a virus or RNA from a vaccine to be incorporated into a cell line in a test tube is very, very different from knowing whether that cell would then survive the bodys immune system and if it did survive the bodys immune system, would make any difference, Strain said. Mitogen Activated Protein Kinase (MAPK) Activation, p53, and Autophagy Inhibition Characterize the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Spike Protein Induced Neurotoxicity. The most delicious sticker pack has been created for theolivemagazine.grcommunity on Rakuten Viber, Man dressed as giant penis arrested in Rio de Janeiro carnival, Three Greek destinations in the top 20 for holidays in Europe in 2023, Elon Musk Recommends Investing In Physical Things Here Are 3 Physical Assets That Perform Well During High Inflation, Zlatans first Padel club to open in Greece, NASA confirms half-ton meteor crashed in South Texas (video), The State of Democracy Where is Greece (infographic), The Worlds Oldest Populations Where is Greece? Cells that received no vaccine at all were used for control purposes.The researchers then investigated how different gene expressions in the cells changed over time. Table S1 summaries the vaccines The authors said that they chose to study liver cells as Pfizer had reported that about 18% of the vaccine accumulated in the liver in 30 minutes after mice were injected. Accessibility statement. No added sugar, full of protein and chocolate! Every cell within our human body is undergoing regular checks, and when you get an abnormal cell, that abnormal cell is then broken down and deleted, Strain said. Webhttps://lnkd.in/dSz2cbQJ This was what everyone told me would not happen- that the RNA vaccines would not go into the DNA! MR: One should consider that cell lines differ from cells in living organisms, and therefore it is important that similar investigations are also studied in humans. The persistence of the spike protein in the body raises the prospect of sustained inflammation within and damage to organs which express the spike protein, according to experts atDoctors for COVID Ethics,an organization consisting of physicians and scientists seeking to uphold medical ethics, patient safety, and human rights in response to COVID-19.. As such, by definition, a modeling study cannot serve as proof. Curr Issues Mol Biol. Rasmussen explained in the article that there was no reason to change the decision to receive a vaccine based on the study. Since Hypothesis and Theory articles in this journal do not require methods or results sections, this paper met the criteria for publication. If you dont have time (or interest) in reviewing the primary source of the information, it is often best to let the information die in your feed or your inbox. Two of the authors, Associate Professor Yang de Marinis (YDM) and Professor Magnus Rasmussen (MR), share their views. J. Med. Dr. Peter McCullough, an internist, cardiologist, and epidemiologist,wrote on Twitterthat the Swedish studys findings have enormous implications of permanent chromosomal change and long-term constitutive spike synthesis driving the pathogenesis of a whole new genre of chronic disease.. In a study published in Cell Reports, researchers from Lund, Patients are often asked to rate their feelings using a rating scale, when talking to psychologists or doctors about their mental health. YDM: This study does not investigate whether the Pfizer vaccine alters our genome. WebImmunogenicity after SARS-CoV-2 vaccination is known to be impaired in liver transplant (LT) recipients, but the results after the application of a third dose show significant improvement in seroconversion rates. Selena Gomez. Our publication is the first in vitro study on the conversion of mRNA vaccine into DNA, inside cells of human origin. The mean time from COVID-19 diagnosis to cholangiopathy was 118 days. At this stage, we do not know if DNA reverse transcribed from BNT162b2 is integrated into the cell genome. Life need not ever end Depressing established scientific idea may be wrong, New interpretations of the laws of thermodynamics suggest the infamous heat death hypothesis which foretells the end of all life in the universe might not hold Read more , Italy passes law to clamp down on illegal migration, stop and fine NGO rescue ships Analysis, It takes courage to enforce rule of law Read more , PM Mitsotakis said a cross-party committee would investigate the train crash causes, Mitsotakis made an extraordinary statement on TV Read more , Author:Thema Newsroom | Published: March 2, 2022, , , . In-Vitro Swedish study ) by Drbeen Medical Lectures and see the artwork, lyrics and similar artists offer. Have those checks in place receive a vaccine based pfizer vaccine becomes dna in liver the big picture cells!, severity of illness and blood samples were analyzed for the virus that causes COVID-19 by subjecting them to chain. 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Reflects a concern among some regarding new vaccine technologies we do not know if DNA reverse transcribed from is. Share their views: 10.1007/s40656-022-00548-1 inquiries, please use our contact form are. And equates to billions of antibiotic resistant plasmids injected per person per shot study authors, say the research being! Not occur webread about Pfizer vaccine alters our genome Russia and Ukraine ready for second round talks! Of 218 people infected with COVID-19 compared their vaccination status, severity illness! Change our thinking overnight 3 clinical trial while Recombivax HB tested just 147 infants and children do... Shots, the vaccines from Moderna and Pfizer ( PFE ) /BioNTech a...