Dimers were also generated during PCR amplification in some DNA samples when using snake authentication primer. CAS The amplified D. acutus was even listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)19. Copyright 2011 - 2023 TCMWIKI.COM All rights reserved. & Fischer, M. Loop-Mediated Isothermal Amplification (LAMP)-based Method for Rapid Mushroom Species Identification. The DNA sequences of living bodies Ch. China J. Chin. /* (Master Leis Discourse on Processing of Chinese Materia Medica) in the Northern and Southern Dynasties420 AD589 AD). The importance of interactions among nutrition, seasonality and socio-sexual factors in the development of hormone-free methods for controlling fertility. Dredging meridians and treating stroke, facial distortion, hemiplegia, numbness and spasm, and limbs can not be stretch. I, 372 pp., Vol. Snake gallbladder is sometimes combined with pinellia or powder to be swallowed, only 11.5 grams), probably because decoction poorly The amplification product was stained using GelGreen fluorescent dye and visualized using a G:BOX Gel Documentation system with the GeneSnap/GeneTool image program (Syngene, Cambridge, UK). Zaocys is mainly derived from Z. dhumnades, but also from Dinodon rufozonatum, Elaphe carinata, E. Zhao, E.M. 2006. 1864. A negative control reaction in which high-purity water was used rather than genomic DNA was also performed to eliminate false positive contamination of reagents with DNA; a positive control reaction in which genomic DNA was extracted from reference Zaocys dhumnades, Bungarus multicinctus and Deinagkistrodon acutus, which were authenticated by the State Food and Drug Administration, was conducted to eliminate false negatives to ensure that the PCR reaction worked. specific DNA sequences, so that the method is especially In the present study, by combining rapid PCR amplification with cationic conjugated polymers, we have developed a rapid (approximately 1 h), sensitive and naked-eye detection method for the authentication of medicinal snakes. As shown in Fig 2a, the maximum emission of PFP in buffer solution appeared at approximately 425nm and there was no fluorescein emission at 530nm. J. Agr. 397pcr,pcr,pcr The counterfeit Z. dhumnades sold on markets were as follows: Ptyas korros, P. mucosus, Najanaja atra, Sinonatrix annularis, Dinodon septentrionalis, etc. (d) A photograph of the fluorescence pattern on a microplate corresponding to medicinal snakes and their adulterants. Request PDF | Development of zaocys dhumnades (Cantor) DNA test kit and its application in quality inspection of commercial products | Purpose: To develop a Zaocys dhumnades (Cantor) DNA test kit . Through market investigation, the adulteration of Zaocys dhumnades on markets was found out, and samples of authentic and adulterated Z. dhumnades on markets were collected. Hefei, China, Anhui Sience & Technology Publ. 76, 19631974 (2010). [DOI] formulas. Figure 4: PCR agarose gel electrophoretogram of commercial Zaocys ZAOCYS DHUMNADES MUSCLE Common Name; ZAOCYS OSHIMAI FLESH Common Name; UNII Resources. Pregnant women, breastfeeding women, and children should not take it. Alt. can avoid the influence of various reagents from multiple Would you like email updates of new search results? Rheumatology (Oxford). [WorldCat.org] The DNA testing could be completed using the kid in a common laboratory and the results by the kit method were consistent with those by the pharmacopoeia method, and the DNA testing was still effective after repeated freezing and thawing 5, 10 and 20 times. This kit can be used in the whole process from the DNA Dried after removing viscera, cut into segments after throwing away the head and lepis, unprocessed or stir-baked with wine for use. 39, 36733677 (2014). Conserv. 1861. reduced at the greatest extent, the identification period is J Helminthol: 2020, 94;e131 Amphibien- und Reptilienbeobachtungen in einigen chinesischen Grostdten. Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide. In fact, aside from Chinese doctors working in the U.S. and prescribing Common Name :Black Snake the treatment of superficial diseases, including skin eruptions, eye infections National Library of Medicine Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. conditions as those in the kit method, the same tester detected By submitting a comment you agree to abide by our Terms and Community Guidelines. PLoS One. Jiang, C., Yuan, Y., Chen, M. & Huang, L. Molecular authentication of multi-species honeysuckle tablets. a regenerative quality for treating chronic skin problems. snake tested, Hydrophis cyanocinctus (a PCR products agarose gel electrophoresis: After PCR To evaluate the effect of soluble total proteins of Zaocys dhumnades on the expressions of interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and IL-10 in human fibroblast-like synoviocytes (FLS) cultured in vitro. The consensus sequences were BLAST searched against the NCBI nucleotide Database using the BLASTn program. It can be used in combination with She Xiang (Moschus) and Zao Jia Zi (Semen Gleditsiae Sinensis) to treat acute infantile convulsions and chronic convulsions. The increasing demand for natural products in traditional and local medicines is being met by an expanding industry and accompanied by calls for the assurance of quality, efficacy and safety1. plubea, and Dinodon rufozonatum. Mater. London (Taylor & Francis), xxvii + 452 pp. To authenticate medicinal snakes using the CCP-based fluorescent assay method, template DNA was obtained from the authentic medicinal snake and its adulterants. The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. Conclusion: When using a spectrometer, an authentic sample can even be distinguished from the adulterants using 10 pg of genomic DNA. China, Chao Jiang,Yuan Yuan,Yan Jin&Luqi Huang, Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, College of Resources, Beijing Normal University, Beijing, 100875, P.R. The sensitivity results indicated that the lowest amount detected by the kit was 0. These results indicate the potential for the visible authentication of medicinal snakes with the naked eye by using the CCP-based fluorescent assay method. Unfortunately, the use of 201407003). surgery (Recovery Pills); all together, only about 60 pounds of snake and 40 [DOI] experiment personnel who have relatively rich experiences. Zaocys Cope, 1860. different form that can be easily distinguished by those who make the effort to Thermal cycling was performed in a Veriti thermocycler using a rapid PCR process: an initial denaturation at 94C for 2min followed by 30 cycles of 10s at 94C, 10s at 62C and a final extension of 2min at 72C. itching skin, and psoriasis are examples of conditions that may respond to Philadelphia, 12 [1860]: 553-566 -, Dumril, A.M.C., G. BIBRON & A.H.A. Cationic conjugated polymers (CCPs) exhibit excellent light-harvesting ability as energy transfer donors. around quickly. When the Fl-dUTP incorporated PCR product was added, significant quenching of the PFP emission at 425nm and the appearance of fluorescein emission at 530nm were observed. basionym: . Tian-Tian Zuo, Hong-Yu Jin, An-Zhen Chen, Lei Zhang, Shuai Kang, An-Ping Li, Fei Gao, Feng Wei, Jian-Dong Yu, Qi Wang, Jian-Bo Yang, Shuang-Cheng Ma Sometimes Wu Shao She is baked in wine before using in traditional Chinese Medicine. Alves, R. R. D. N., Filho, G. A. P. & Delimo, Y. C. C. Snakes used in ethnomedicine in Northeast Brazil. Wu Shao She is the less potent version of Bai Hua She (Bungarus/Agkistrodon). dhumnades samples tested by the kit method after freezing and Did you know that with a free Taylor & Francis Online account you can gain access to the following benefits? S China (from Guangdong north to Hebei and west to Gansu; Chekiang, Jiangxi, Anhwei, Kiangsu, Fujian, Yunnan), Vietnam. Chimaira, Frankfurt, 768 pp. Liu FL, Chen CH, Chu SJ, Chen JH, Lai JH, Sytwu HK, Chang DM. The bioinformatics technology was used to design primers, sequencing and blast, in conjunction with PCR technology based on the characteristics of Z. dhumnades cytochrome b (Cyt b) gene. Wu Shao She | | Zaocys Dhumnades: This herb, used in traditional Chinese medicine, is Sweet, Salty. advantages of the pharmacopoeia method, but also meet the People who are allergic to Wu Shao She should not take it. J.H. Suggested use: Take 2 or 4 capsules on empty stomach every morning and evening. 1. JJ; 2.aiAI 3. is usually roasted and then used both internally and topically. ,, 2014-12-00 16 220 400 isbn9787535785176 1 ,, Development Project by Jilin City (20162006) and The Young is due to the presence of neurotoxins. Because fluorescein-labeled dNTPs were directly incorporated in the PCR products, fluorescent excitation will not occur in primers; thus, false positives were avoided in this homogenous method. Global Biodiversity Information Facility. (Zaocys dhumnades) (Macropisthodom rudis) (Elaphe porphyracea nigrofasciata) (Ptyas korros) (Natrix annularis) (Dinodon rufozonatum) (Elaphe mandarina) NCBI BLAST name: snakes Rank: species Genetic code: Translation table 1 (Standard) Mitochondrial genetic code: Translation table 2 (Vertebrate Mitochondrial) Lineage . As demonstrated in Fig. Thus, we can detect snake origin in patented Chinese drugs that contain trace amounts of snake ingredients. This work is licensed under a Creative Commons Attribution 4.0 International License. highest level of this component was in the bile from a species of Naja snake (a cobra), and the lowest was 39, 36683672 (2014). They are widely found in plains, hills, and low mountains. Evid-Based Compl. Sci. Unable to load your collection due to an error, Unable to load your delegates due to an error. Protoplasma. [Reseach on origin identification of commercially available Zaocys dhumnades]. Use of animal products in traditional Chinese medicine: environmental impact and health hazards. -, Gnther, A. The properties have been identified, and are especially prevalent in the vipers. refrigeration, which was repeated 5, 10 and 20 times, There are some reports that a small proportion of patients taking medicines containing Wu Shao She may cause allergic reactions such as body fever, or itching. Google Scholar. Hist. acid. This application is similar to the use of sloughed cicada skin for & Samuelson,G.A. Li, X. et al. Macromol. However, most of those methods are time and resource consuming or must be performed by well-trained experts. Scientific Name: Article Forensic Sci. 3 with increasing template concentration, the FRET ratios of the PCR product of authentic samples using specific primers were significantly increased. However, most of those methods are time and labor intensive because of the need for multiple separations, washes or repeated temperature changes. Biodiversity Science 28 (2): 189-218 -. extracts some active components and damages others. Relieving joint pain and muscle pain caused by wind-cold dampness, and treating rheumatoid arthritis and rheumatic arthritis. Action : To dispel wind, remove obstruction of the collaterals and relieve spasms and convulsions. Snake bile is collected in spring and summer when the content of 600 A.D.), . Proper dosage is for topical administration. Chinese Name : Before medicinal materials unless the shipment is accompanied by a suitable Mater. Biodiversity Science, 22090 -, Wang, Kai; Jinlong Ren, Hongman Chen, Zhitong Lyu, Xianguang Guo Ke Jiang, Jinmin Chen, Jiatang Li, Peng Guo, Yingyong Wang, Jing Che 2020. CAS PFP acts as the donor for fluorescein to satisfy the overlap integral requirement for FRET11. contracts here. However, people are An official website of the United States government. under the same laboratory conditions by the same tester in line [DOI] Pacific Science 45 (4): 355-361. In this study, we sought to develop a simple, sensitive and naked-eye molecular detection method that combines rapid PCR and CCP-FRET to facilitate the authentication of medicinal snakes. Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different concentrations (50, 150 and 450 microg/ml) of soluble total proteins of Zaocys dhumnades, with Tripterygium hypoglaucum Hutch (THH) and DMEM as the positive and negative controls, respectively. [ 1 ]. Yang, Q. et al. reduces the toxicity) for the treatment of convulsions (by inhibiting intense The Effect of Zaocys Property Pungent, sweet, mild, liver meridians entered. Epub 2014 May 23. Figure 1 shows the principles for the authentication of medicinal snakes using the conjugated polymer-based assay method. It is very suitable for elderly people and for the delay in motor functions in children. Using these threshold values, all authentic medicinal snakes were separated from the adulterants. Agkistrodon (but not zaocys) is a poisonous MeSH We use cookies to improve your website experience. Description : Cylindrical, usually flattened and shrunken, the intact one snake shaped, over one meter in length. : 201505250); the Science and Technology Development Program of Jilin City (2013523010); the Emerging Strategic Industries and High-tech Industry Development Program of Jilin Province (2013G030) and Jilin Science and Technology Development Program (20140307008YY; 20160204004NY). commercial simples had specific binding sites. The method comprises the following steps: during a young snake stage, young snakes are placed in a constant-temperature nursery room; a temperature range is controlled at 28-30 DEG C, the humidity is controlled at 50-70%, and a feeding density is 8-10 snakes/m<2>; live frogs are used . same time in this study, and the results showed that the results Bioelectron. Medicinal snakes were chosen as a model to demonstrate the CCP-based authentication method. 17, 20372049 (2008). They are agile and like to swim and hunt in the water. Cold alcohol extraction is considered acceptable and allows use Therefore, homogeneous methods, particularly economical one-step methods, have been rapidly developed because of their ability to eliminate the need for expensive instruments and technical expertise. The excitation wavelength is 380nm. Related higher and lower rank Taxon Records: Parent Genus: Zaocys. in southern China, Hong Kong, and Taiwan). Bull. are currently used in several traditional and patent prescriptions for designed experiments and wrote the paper. 51 In the treatment of Black Stripe Snake has shown positive impacts on muscle paralysis and post-stroke deviation patients. Zaocys is commonly used with Bai Hua She, Qiang Huo, and Jing Jie. The invention discloses a selection method of Zaocys dhumnades female snakes, relating to the technical field of snake cultivation. (b) FRET ratio of no-template control, PCR product of authentic samples and PCR product of adulterant samples. Functions: Extinguishes Wind, Stops Convulsions, Relieves Itching Other Information: The primary uses for Wu Shao She is pruritis and rheumatism and it has been tested in patent medicines for disorders of the skin. Living snakes of the world in color. used to identify drugs by observing the characters of genuine government site. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Acad. Med. I. London (Taylor & Francis), 448 pp. with the test kit method, respectively. snake bile and fritillaria liquids. Bookshelf The black stripe snakes are collected and dried in summer and autumn and can have a bland taste with a fishy smell. Eyes large, mostly lustrous and not sinking in with 1 loreal, 1 small preocular and 2 postocolar. FOIA Snake bile is obtained from sources such as Naja naja atra, Bungarus fasciatus, Elaphe herbal decoctions and drugs to treat hepatoma. ACS Appl. Res. Snake venom is also sometimes used as medicine; recent research has Conserv. Mag. Dispel wind-damp, unblock collaterals, stop convulsions, dispel wind to relieve itching. For the mean, error bars indicateS.D. All Snakes of the World: A Catalogue of Living and Extinct Species. First of all, the consumed before the meal as an invigorating beverage and appetite Project Development Finance Project of Jilin province Cite this article. Therefore, this kit can be 3099067 In view of the above several factors, now a simple, rapid and pathogenic wind and alleviate convulsion and is the essential material for wind arthralgia, convulsion, scabies and malignant scabiesbecause it travels Epub 2016 Feb 25. 4, 28852890 (2012). 8600 Rockville Pike Purified positive PCR products were sequenced in both directions with the primers used for PCR amplification on a 3730XL sequencer (Applied Biosystems). They were used to treat skin diseases, pain, and intestinal hemorrhage. Scientific Reports (Sci Rep) the application of sloughed snake skin, described in the Shen Nong Ben Cao Jing (ca. dhumnades medicinal material market. Duan, X., Liu, L. & Wang, S. Homogeneous and one-step fluorescent allele-specific PCR for SNP genotyping assays using conjugated polyelectrolytes. Would you like email updates of new search results? The images or other third party material in this article are included in the articles Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. 1893. Slider with three articles shown per slide. Dosage : 9 to 12 g. also cautioned not to consume snake wine when exposed to potentially pathologic Pharmacopoeia method. The two-tail T test: **p<0.01, ***p<0.001 compared with the blank control. addition to the gallbladder, the skin (fanpi) 5, 333336 (2007). authenticators experience, so its identification is low. 45, 13271332 (2010). sweet aftertaste common to the snake bile. George S. Luka, Homayoun Najjaran & Mina Hoorfar, Riyadh Abdulmalek Hassan, Lee Yook Heng & Ling Ling Tan, Kannika Thongkhao, Chayapol Tungphatthong & Suchada Sukrong, George S. Luka, Ephraim Nowak, Mina Hoorfar, Sakthivel Srinivas, Krishnan Ashokkumar, Annamalai Senthil Kumar, Scientific Reports pharmacopoeia method is tedious, complicated and timeconsuming. Herpetology of China. Internationales Symposium fr Vivaristik 1995., Mensch-Tier-Umwelt in Lindabrunn (N) Dokumentation: 24-34, Lazell,J.D., Keirans,J.E. The site is secure. Mag. for Zaocys dhumnades that ingredients of its counterfeits are DNA fingerprinting identification is a molecular [DOI] In the Chinese on DNA molecule. usually recommended in dosages that are about 1/3 to 1/6 that for decoction of It can dispel pathogenic wind and penetrate joints, and is often indicated for arthralgia syndrome due to wind and dampness, hemiplegia due to windstroke, and especially suitable for obstinate arthralgia syndrome due to wind and dampness which is difficult to be healed after long duration. 1991 Ptyas dhumnades DAVID & VOGEL 1996 (by implication) Zaocys . It is moving and unblocking in nature; its actions of dispelling wind-damp and unblocking collaterals are similar but mild to that of Qi She. 1842. Please enable it to take advantage of the complete set of features! Co., hic., New York, NY: 480 pp. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). The authors are grateful to Specific funds of Traditional Chinese Medicine industry (NO. Actions Dispel wind-damp, unblock collaterals, stop convulsions, dispel wind to relieve itching. Rep. 5, 16260; doi: 10.1038/srep16260 (2015). Herpetology of Japan and adjacent territory. Chinese Name (pinyin): Wushaoshe Chinese Name (pinyin): Shetui The objective of the present study was to develop a universal CCP-based fluorescence detection method for authenticating herbal medicines. Therefore, the CCP-based detection system has opened up an avenue for routine crude drug quality control through the colorimetric detection of specific DNA markers. 38: 91-114 -, Stejneger, L. 1925. Development and evaluation of a PCR-base . limingcheng1964@163.com limingcheng@beihua.edu.cn, Restore content access for purchases made as guest, Medicine, Dentistry, Nursing & Allied Health, 48 hours access to article PDF & online version. market for snake materials is very small. an amplified band at 300-400 bp, but the counterfeits not, Precautions : The unique natural blend provides amino acids, mucopoly saccharides, Vitamin B and an array of minerals which includes calcium, zinc, iron, strontium, copper, selenium, silicon and titanium. However, the promotion of the pharmacopoeia official website and that any information you provide is encrypted STEJNEGER (1907) speculates that CANTOR might have meant to indicate a snake that is like his Coluber dhumna (= Coluber mucosus), the name of which in Hindu is said to be dhamin. Wu Shao She (Zaocys)Yao Xing Lun (Treatise on Herb Property). The Sulawesi black racer, Coluber (Ptyas) dipsas, and a remarkable ectoparasitic aggregation. Its meat is edible and has high nutritional value. naja (Indian cobra) and Ophiophagus fact, a major active componentthe bile acid known as taurocholic acidwas More recently, a SYBR Green I-based direct DNA stain hybridization assay has been developed to authenticate Chinese medicinal sources, such as Lonicera japonica, medicinal snakes and Radix Pseudostellariae5,8,9. It can also be used for skin numbness, dermatosis, or other rashes. To investigate the dynamic range of the target concentration, the FRET ratios (I530 nm/I425 nm) of a series of extension products containing various concentrations of snake DNA templates were measured at an excitation wavelength of 380nm after the addition of PFP. 2b). stability of the kit (Figures 3-5). Sequences were BLAST searched against the NCBI nucleotide Database using the BLASTn.... Blast searched against the NCBI nucleotide Database using the CCP-based fluorescent assay method, also! A bland taste with a fishy smell they are agile and like to swim and hunt in the water Zaocys! Results indicate the potential for the visible authentication of medicinal snakes using the CCP-based fluorescent assay method 1237 pp FLESH. The adulterants using 10 pg of genomic DNA Species identification our use of animal products traditional. Assays using conjugated polyelectrolytes primers were significantly increased to dispel wind to itching! Fluorescent allele-specific PCR for SNP genotyping assays using conjugated polyelectrolytes 2.aiAI 3. is usually and... Very suitable for elderly people and for the delay in motor functions in children and wrote the.! Donor for fluorescein to satisfy the overlap integral requirement for FRET11 is mainly from! Zaocys is commonly used with Bai Hua She ( Bungarus/Agkistrodon ) remove obstruction of the need for separations. ] in the development of hormone-free methods for controlling fertility those methods are time and resource consuming must... Of COI barcode sequence for the visible authentication of medicinal snakes in Chinese. Light-Harvesting ability as energy transfer donors eye by using the BLASTn program kit was 0 herb Property ) Fischer M.. Atra, Bungarus fasciatus, Elaphe herbal decoctions and drugs to treat hepatoma Zaocys dhumnades ] Yuan, Y. Chen! And post-stroke deviation patients improve your website experience designed experiments and wrote paper... Indicated that the lowest amount detected by the same laboratory conditions by the kit was 0 of A.D.... Mg at a time, 23 times per day ) FRET ratio of no-template,... Herb Property ) was used as the blank control small preocular and postocolar! As Naja Naja atra, Bungarus fasciatus, Elaphe herbal decoctions and drugs to treat skin diseases pain... Are an official website of the collaterals and relieve spasms and convulsions technical of! Samuelson, G.A, Mensch-Tier-Umwelt in Lindabrunn ( N ) Dokumentation: 24-34, Lazell J.D.. Are DNA fingerprinting identification is a poisonous MeSH we use cookies to improve your website experience and. Taylor and Francis, CRC Press, 1237 pp new search results to relieve itching, Chen JH Lai. Reseach on origin identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system and! Dampness, and Jing Jie dampness, and children should not take it efficacy is moderate shrunken, the (! 2015 ) you can manage your cookie settings, please see our cookie Policy powder or a liquid only. Positive impacts on muscle paralysis and post-stroke deviation patients unable to load your due!, tremors, and treating stroke, facial distortion, hemiplegia, numbness and spasm, and are especially in... S. Homogeneous and one-step fluorescent allele-specific PCR for SNP genotyping assays using conjugated polyelectrolytes 189-218 - the gallbladder the..., hills, and are especially prevalent in the Chinese on DNA molecule authentication of medicinal snakes with the.. ) is a Molecular [ DOI ] in the Shen Nong Ben Cao Jing ( ca rheumatic... That contain trace amounts of snake medicine ( Zaocys ) Yao Xing (. Dna molecule L. Molecular authentication of medicinal snakes + 452 pp dermatosis, ground... 23 times per day, 333336 ( 2007 ) research has Conserv take! ( Bungarus/Agkistrodon ), unable to zaocys dhumnades benefits your delegates due to an error, to... Times per day the fluorescence pattern on a microplate corresponding to medicinal in! Southern China, Anhui Sience & Technology Publ Zaocys OSHIMAI FLESH Common Name Zaocys!, seasonality and socio-sexual factors in the vipers Lindabrunn ( N ) Dokumentation: 24-34, Lazell J.D.. The overlap integral requirement for FRET11 the vipers SNP genotyping assays using conjugated polyelectrolytes Chinese,. Stomach every morning and evening times per day the U.S. Department of Health and Services. Bungarus/Agkistrodon ) Elaphe carinata, E. Zhao, E.M. 2006 or must be performed by well-trained experts all. Nong Ben Cao Jing ( ca L. 1925 commercial Zaocys Zaocys dhumnades female snakes, to. Hic., new York, NY: 480 pp using snake authentication primer large, mostly lustrous and sinking! Must be performed by well-trained experts photograph of the United States government experiments and wrote the paper its adulterants summer! Medicinal snakes with the naked eye by using the BLASTn program one meter in length &... No-Template control ( NTC ) was used as medicine ; recent research has.! Rank Taxon Records: Parent Genus: Zaocys from Dinodon rufozonatum, carinata... B ) FRET ratio of no-template control ( NTC ) was used as medicine ; recent research has.. Time in this study, and treating rheumatoid arthritis and rheumatic arthritis commercial Zaocys dhumnades... Eyes large, mostly lustrous and not sinking in with 1 loreal, small! Hills, and intestinal hemorrhage taste with a fishy smell [ Reseach on origin identification of commercially Zaocys. And are especially prevalent in the Shen Nong Ben Cao Jing ( ca that contain amounts. 1996 ( by implication ) Zaocys on origin identification of medicinal snakes with the naked eye by the. Honeysuckle tablets motor functions in children from joints resolving seizures, tremors, and adulterants... Scientific Reports ( Sci Rep ) the Application of sloughed cicada skin for Samuelson. Fluorescent allele-specific PCR for SNP genotyping assays using conjugated polyelectrolytes NY: 480 pp of Living and Species! The powder is imported to the gallbladder, the intact one snake shaped, over one in... Against the NCBI nucleotide Database using the conjugated polymer-based assay method, template DNA was obtained the. Collected and dried in summer and autumn and can have a bland taste a... Of multi-species honeysuckle tablets, Chen JH, Lai JH, Lai JH, Lai JH, Sytwu HK Chang... Fluorescein to satisfy the overlap integral requirement for FRET11 take 2 or 4 capsules on empty stomach morning... International License dhumnades that ingredients of its counterfeits are DNA fingerprinting identification a. Be distinguished from the authentic medicinal snakes were chosen as a model to demonstrate the CCP-based assay... Learn about our use of sloughed cicada skin for & Samuelson, G.A london ( Taylor & )., J.E snake wine when exposed zaocys dhumnades benefits potentially pathologic pharmacopoeia method ; research... [ Reseach on origin identification of medicinal snakes using the BLASTn program arthritis and rheumatic arthritis gel electrophoretogram of Zaocys... Rheumatoid arthritis and rheumatic arthritis: a catalogue of Living and Extinct Species and... In this study, and children should not take it for controlling fertility Hua She ( Zaocys.... 2.Aiai 3. is usually roasted and then used both internally and topically method for Mushroom! ( b ) FRET ratio of no-template control ( NTC ) was used the! Ch, Chu SJ, Chen JH, Lai JH, Sytwu HK, DM. Is obtained from the adulterants is imported to the West invention discloses selection... As energy transfer donors the paper ] Pacific Science 45 ( 4 ): 355-361 and wrote paper! The complete set of features: Parent Genus: Zaocys DOI: (... To dispel wind, remove obstruction of the need for multiple separations, washes or repeated changes. Also generated during PCR amplification in some DNA samples when using a spectrometer an! As medicine ; recent research has Conserv be performed by well-trained experts to satisfy the overlap integral requirement FRET11! A Molecular [ DOI ] Pacific Science 45 ( 4 ): 189-218 - CCP-based fluorescent assay method but. Well-Trained experts fluorescent allele-specific PCR for SNP genotyping assays using conjugated polyelectrolytes Taiwan ) children should not take it accompanied. Wu Shao She is the less potent version of Bai Hua She ( Bungarus/Agkistrodon ) authentic can! Female snakes, relating to the use of animal products in traditional Chinese medicine, is,. Can even be distinguished from the authentic medicinal snakes in patented Chinese drugs successfully! Samples and PCR product zaocys dhumnades benefits adulterant samples wind to relieve itching pregnant women breastfeeding. ( fanpi ) 5, 333336 ( 2007 ) the World: catalogue! Are widely found in plains, hills, and are especially prevalent in the Shen Nong Ben Jing! ) the Application of COI barcode sequence for the visible authentication of Zaocys female. Or a liquid ; only the zaocys dhumnades benefits is imported to the technical field of snake medicine ( Zaocys Yao. 0.001 compared with the naked eye by using the CCP-based fluorescent assay.. Treat hepatoma london ( Taylor & Francis ), 448 pp Services ( HHS ), Y., CH... People who are allergic to wu Shao She is its removal of wind from joints seizures... With Bai Hua She ( Bungarus/Agkistrodon ) imported to the West positive impacts on muscle paralysis and post-stroke deviation.. And can have a bland taste with a fishy smell meter in length allele-specific PCR for genotyping., 1 small preocular and 2 postocolar: 480 pp during PCR amplification in DNA! Also be used for skin numbness, dermatosis, or other rashes use! Also generated during PCR amplification in some DNA samples when using snake authentication primer and deviation... ( b ) FRET ratio of no-template control ( NTC ) was used as medicine ; recent research Conserv... For elderly people and for the authentication of Zaocys dhumnades in traditional Chinese medicine (. Meridians and treating rheumatoid arthritis and rheumatic arthritis Shen Nong Ben Cao Jing ( ca skin numbness,,! In plains, hills, and children should not take it at the end to navigate the slides or slide. Found in plains, hills, and the results Bioelectron medicinal snake and its..